Coding

Part:BBa_K1670006:Design

Designed by: Magdalena Hannah Kurteu   Group: iGEM15_Manchester-Graz   (2015-09-15)


Aromatic amino acid decarboxylase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 30
    Illegal BglII site found at 80
    Illegal BamHI site found at 3
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1384
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original sequence was modified via PCR mutagenesis in order to remove an internal PstI restriction site.


Source

The sequence was obtained from the genomic sequence of Enterococcus faecium

References